Study: Bacterial and fungal isolation found in face masks


The COVID-19 pandemic has led people to wear face masks daily in public. Although the effectiveness of face masks against viral transmission has been extensively studied, there have been few reports on potential hygiene issues due to bacteria and fungi attached to the face masks. We aimed to (1) quantify and identify the bacteria and fungi attaching to the masks, and (2) investigate whether the mask-attached microbes could be associated with the types and usage of the masks and individual lifestyles. We surveyed 109 volunteers on their mask usage and lifestyles, and cultured bacteria and fungi from either the face-side or outer-side of their masks. The bacterial colony numbers were greater on the face-side than the outer-side; the fungal colony numbers were fewer on the face-side than the outer-side. A longer mask usage significantly increased the fungal colony numbers but not the bacterial colony numbers. Although most identified microbes were non-pathogenic in humans; Staphylococcus epidermidis, Staphylococcus aureus, and Cladosporium, we found several pathogenic microbes; Bacillus cereus, Staphylococcus saprophyticus, Aspergillus, and Microsporum. We also found no associations of mask-attached microbes with the transportation methods or gargling. We propose that immunocompromised people should avoid repeated use of masks to prevent microbial infection.


The rapid global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the resulting coronavirus disease 2019 (COVID-19) pandemic have led to urgent efforts to prevent the viral transmission. The most traditional and reasonable method to prevent respiratory infections is to wear face masks; several research groups have demonstrated its effectiveness against the respiratory viral transmission before the COVID-19 pandemic1,2. During the COVID-19 pandemic, increasing lines of evidence have supported the effectiveness of wearing face masks against SARS-CoV-2 and the droplets3,4. However, the World Health Organization (WHO) claims that face masks are effective only when used with hand hygiene, the proper use, and disposal of masks5.

Three types of face masks are commercially available for daily lives in Japan: (1) non-woven, (2) polyurethane, and (3) gauze or cloth masks (Fig. 1a,b). Non-woven masks are commonly used worldwide to prevent droplet infections by most respiratory microbes, including SARS-CoV-2 (Fig. 1c). Polyurethane masks have been used to protect against hay fever, particularly in Asian countries. Since polyurethane masks are easy to breathe and washable, the masks have become popular and have been reused several times during the COVID-19 pandemic. Although gauze masks are less popular, the masks can be washed, reused, and effectively prevent infections. Thus, the Japanese government distributed gauze masks to all citizens because of the shortage of non-woven masks during the early stage of the COVID-19 pandemic.